PCR Master Mix Example for a 25 uL Reaction
Worked example for setting up a 25 uL endpoint PCR reaction with overage and control wells.
Best for
Researchers preparing routine endpoint PCR or first-pass assay validation.
Open exampleWorked Examples
Real experiment setups, not just generic calculators
These worked examples show how a researcher would actually use BioLT during PCR planning, cell seeding, cloning, and qPCR interpretation. Each page is meant to stand on its own as reference content.
10-fold Serial Dilution Example for a Standard Curve
Worked example showing how to build a standard curve dilution series without carryover mistakes.
Best for
Users preparing qPCR standards, titration panels, or assay sensitivity checks.
Open exampleCell Seeding Example for a 6-well Plate
Worked example for turning a live cell count into a practical seeding plan for a 6-well experiment.
Best for
Researchers seeding mammalian cells for routine culture or treatment assays.
Open exampleHemocytometer and Trypan Blue Example
Worked example for converting manual chamber counts into viable cell concentration and viability.
Best for
Researchers who still rely on manual counting and need a clean reference for the math.
Open exampleLigation Ratio Example for Insert and Vector Planning
Worked example for choosing insert amount from vector mass, fragment length, and target molar ratio.
Best for
Researchers setting up routine cloning reactions and trying to avoid mass-ratio mistakes.
Open exampleqPCR ddCt Interpretation Example
Worked example for turning Ct values into dCt, ddCt, and fold-change with interpretation notes.
Best for
Researchers using relative expression analysis and checking whether a result is interpretable.
Open exampleA260 Purity Check Example Before PCR
Worked example for deciding whether a nucleic acid prep is clean enough for downstream use or needs cleanup first.
Best for
Researchers checking whether a DNA or RNA prep is ready for PCR, cloning, or qPCR.
Open exampleCopy Number Example for qPCR Standard Preparation
Worked example for converting DNA mass into copies and preparing a standard series with enough volume for replicates.
Best for
Researchers building plasmid or amplicon standards for qPCR or assay sensitivity checks.
Open exampleTransformation Efficiency Example After Cloning
Worked example for calculating CFU per microgram and interpreting whether a low-colony outcome reflects competent cells, ligation, or plating dilution.
Best for
Researchers reviewing a transformation result after ligation or control plasmid transformation.
Open exampleRCF to RPM Conversion Example for a New Rotor
Worked example for converting a published spin force into the RPM needed on a centrifuge with a different rotor radius.
Best for
Researchers adapting a protocol from a paper, kit, or another lab onto their own centrifuge.
Open example