Reference Guides

Bench guides that explain the numbers behind each experiment

These pages add context that a calculator alone cannot provide: when to use a method, common interpretation mistakes, practical caveats, and what to check before you trust the output.

The guide library currently includes 10 original reference pages focused on assay planning, interpretation, troubleshooting, and pre-bench checks.

Primer Tm Curation

How to evaluate and compare Tm estimates

Each guide is written to reduce setup mistakes, highlight edge cases, and make the linked tools easier to use in a real lab workflow.

Statistical Test Chooser

Simple decision tree and links

Each guide is written to reduce setup mistakes, highlight edge cases, and make the linked tools easier to use in a real lab workflow.

Buffer pH / pKa

Why buffer compatibility matters

Each guide is written to reduce setup mistakes, highlight edge cases, and make the linked tools easier to use in a real lab workflow.

Osmolarity Curation

Practical checklist for media prep

Each guide is written to reduce setup mistakes, highlight edge cases, and make the linked tools easier to use in a real lab workflow.

A260 Purity Ratios in Practice

How to read A260/280 and A260/230 before using a sample

Each guide is written to reduce setup mistakes, highlight edge cases, and make the linked tools easier to use in a real lab workflow.

Ligation Molar Ratio Planning

Choose insert and vector amounts without falling back to mass ratio shortcuts

Each guide is written to reduce setup mistakes, highlight edge cases, and make the linked tools easier to use in a real lab workflow.

qPCR ddCt Interpretation

Know when fold change is meaningful and when the upstream data are too weak

Each guide is written to reduce setup mistakes, highlight edge cases, and make the linked tools easier to use in a real lab workflow.

Cell Seeding Density Planning

Translate target confluence and assay timing into a practical seeding plan

Each guide is written to reduce setup mistakes, highlight edge cases, and make the linked tools easier to use in a real lab workflow.

Hemocytometer Counting Quality Checks

Reduce counting noise, clump bias, and dilution mistakes in manual counts

Each guide is written to reduce setup mistakes, highlight edge cases, and make the linked tools easier to use in a real lab workflow.

RCF vs RPM for Centrifuge Protocols

Match published spin conditions to your own rotor instead of copying RPM blindly

Each guide is written to reduce setup mistakes, highlight edge cases, and make the linked tools easier to use in a real lab workflow.