Workflow Notes

Step-by-step workflows for everyday lab prep

BioLT is not only a calculator directory. These workflow pages connect several tools into a single experiment sequence so users can understand where each calculation fits in practice.

The workflow library currently includes 7 practical sequences covering quantification, PCR setup, cloning, qPCR standards, cell counting, and plating decisions.

Cell culture routine

Seeding, counting, and media handling checklist

Linked tools

cell-seeding, hemocytometer

PCR and qPCR setup

Master mix balance and control planning

Linked tools

pcr-master-mix, copy-number

Molecular cloning

Ligation molar ratio and dilution checks

Linked tools

ligation, multi-stock-mix

DNA quantification to PCR setup

Move from concentration check to dilution planning and final PCR mix assembly

Linked tools

a260, serial-dilution, pcr-master-mix

qPCR standard curve preparation

Build a standard series, document copy number assumptions, and protect replicate volume

Linked tools

copy-number, serial-dilution, pcr-master-mix

Ligation to transformation sequence

Connect ligation setup to transformation plating and final efficiency review

Linked tools

ligation, multi-stock-mix, transformation-efficiency

Manual count to plating plan

Use hemocytometer data to prepare a mixed suspension and seed plates consistently

Linked tools

hemocytometer, cell-seeding, cell-doubling-time