This workflow connects daily maintenance, counting, and seeding decisions into one repeatable sequence. It is intended for routine mammalian cell culture work where consistency matters more than speed alone.
Steps
- Label plates, flasks, and waste containers before opening the hood.
- Warm complete medium and detachment reagents to the correct temperature.
- Harvest cells gently and resuspend until clumps are minimized.
- Count cells with a hemocytometer and confirm viability assumptions.
- Use the seeding calculator to match plate format, density, and final volume.
- Mix again immediately before dispensing to reduce settling bias.
- Record the actual seeded density, volume, passage number, and unusual observations.
Control points
- Confirm that morphology and contamination status are acceptable before passage.
- Check whether the intended density fits the plate surface area and experiment timeline.
- Add overage for pipetting loss and dead volume instead of improvising at the bench.
Use these tools
- Cell Seeding
- Hemocytometer
Tips
- Pre-fill edge wells when appropriate to reduce evaporation artifacts.
- Do not rely on the initial suspension mix if the dispense takes several minutes.
- Record viability and passage number because both can explain downstream variability.