Cell Seeding Example for a 6-well Plate

You counted cells after harvest and need to seed a 6-well plate at a consistent density.

The value of the example is not only the final number, but also the sequence: count, confirm viability, calculate, then dispense.

Initial concentration from a recent count or hemocytometer result.

Target cells per well or target cells per cm squared depending on the assay design.

Final volume per well and an overage factor for dead volume.

Prepare one mixed suspension instead of recalculating per well.

Mix again immediately before each dispense to reduce settling bias.

Record the final seeded density along with passage number and any notable morphology changes.

Using a cell count collected too early and allowing the suspension to settle before plating.

Forgetting that different plate formats have different growth areas.

Treating viability and total cell count as interchangeable.