Hemocytometer and Trypan Blue Example

You mixed a harvested cell suspension with trypan blue and counted several squares in a hemocytometer.

This example shows how to translate those counts into viable cells per mL and percent viability.

Counted squares and dilution factor.

Separate live and dead counts rather than using one combined number.

Use enough squares to reduce sampling noise.

Manual counting is still common in small labs and during troubleshooting.

A worked example helps newer users catch unit and dilution mistakes that often survive into the actual seeding step.

Using too few squares for a stable average.

Counting clumps as single cells or vice versa.

Forgetting that the dilution factor changes both concentration and viability interpretation.