Cell culture routine
Seeding, counting, and media handling checklist
Linked tools
Cell Seeding, Hemocytometer
Open workflowWorkflow Notes
Step-by-step workflows for everyday lab prep
BioLT is not only a calculator directory. These workflow pages connect several tools into a single experiment sequence so users can understand where each calculation fits in practice.
The workflow library currently includes 12 practical sequences covering quantification, PCR setup, cloning, qPCR standards, cell counting, and plating decisions.
PCR and qPCR setup
Master mix balance and control planning
Linked tools
PCR/qPCR Master Mix, DNA/RNA Copy Number
Open workflowMolecular cloning
Ligation molar ratio and dilution checks
Linked tools
Ligation Setup, Multi-Stock Mixing
Open workflowDNA quantification to PCR setup
Move from concentration check to dilution planning and final PCR mix assembly
Linked tools
A260 Quantitation, Serial Dilution Planner, PCR/qPCR Master Mix
Open workflowqPCR standard curve preparation
Build a standard series, document copy number assumptions, and protect replicate volume
Linked tools
DNA/RNA Copy Number, Serial Dilution Planner, PCR/qPCR Master Mix
Open workflowLigation to transformation sequence
Connect ligation setup to transformation plating and final efficiency review
Linked tools
Ligation Setup, Multi-Stock Mixing, Bacterial Transformation Efficiency
Open workflowManual count to plating plan
Use hemocytometer data to prepare a mixed suspension and seed plates consistently
Linked tools
Hemocytometer, Cell Seeding, Cell Doubling Time
Open workflowRNA handling to qPCR interpretation
Connect RNA quality, reverse transcription, controls, and final Ct interpretation
Linked tools
qPCR Relative Quantification (ddCt), PCR/qPCR Master Mix
Open workflowGel check to cleanup decision
Use gel behavior to decide whether to proceed, repeat, excise, or clean up
Linked tools
Gel Loading Calculator, A260 Quantitation
Open workflowCell treatment plate planning
Plan seeding, treatment timing, controls, viability checks, and plate layout together
Linked tools
Cell Seeding, Hemocytometer, Cell Doubling Time
Open workflowBuffer and media preparation workflow
Plan pH, osmolarity, supplements, labeling, and storage records before use
Linked tools
Reconstitution Helper, Multi-Stock Mixing
Open workflowExperiment review and troubleshooting cycle
Turn calculations, controls, observations, and deviations into a reusable review loop
Linked tools
PCR/qPCR Master Mix, Serial Dilution Planner, Cell Seeding
Open workflow